GC analysis For GC gas chromatography an Agilent Technologies (7890A) interfaced with a mass selective detector (MSD, Agilent 7000 Triple Quad) equipped with an Agilent HP-5ms polar detector ( 5%-phenylmethylpolysiloxane) (J&W Scientific, Folsom, CA, USA) equipped with a capillary column (30 m × 0.25 mm internal diameter and 0.25 μm film thickness) and FID detector. Land (Country) used for the fractionation of date fruits, Echinacea and camel extracts according to the methods performed by Dionex 22. GC-MS analysis The ethanol extracts of the aerial part of Echinacea or of the date pulp fruits were performed separately using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i autosampler and a gas chromatograph interfaced with a mass spectrometer (GC-MS) equipped with an Elite-5MS (5% diphenyl/95% dimethyl polysiloxane) have fused a capillary column (30 × 0.25 μm ID × 0.25 μm df). For GC-MS detection, an electron ionization system was used in electron impact mode with an ionization energy of 70 eV. Helium gas (99.999%) was used as the carrier gas at a constant flow rate of 1 mL/min, and the samples were injected at a split ratio of 50:1; Helium was used as the carrier gas at 1.0 ml/min. The column temperature was held at 100°C for 1 minute after injection, then increased at 10°C/min to 275°C, temperature held for 20 minutes. The time required for chromatography of a sample was 40 minutes. TIC Method for Identification of Constituents Identification and Quantification of Constituents: The relative percentage amount of each component was calculated by comparing the average peak area with the total areas (TIC) using the instrument software. The mass detector used in this analysis was Turbo-Mass Gold-Perkin-Elmer and the software used to manage the mass spectra and chromatograms...... middle of paper......don Scientific and Cheshire, United Kingdom United), sectioned (4μm) and stained with hematoxylin and eosin (H&E). The slides were mounted with neutral conditioner. Sensory evaluation Sensory evaluation of the camel milk, date pulp and echinacea blends was assessed using a standard taste panel (appearance, 5-body and texture, 5-and flavour, 10) by Food Sci staff members. Dept., Fac. of Agric., Ain Shams University according to Clark et al., 26. Works cited46. Al-Farsi M., Alasalvar C., Al-Abid M, et al.: Composition and functional characteristics of dates, syrups and their by-products. Food Chemistry 2007; 104:943-94747. Chaplin DD: Overview of the immune response, J Allergy Clin Immunol 2010;125(2), S3-23.48. Iemoli E, Piconi S, Fusi A, et al.: Immunological effects of Omalizumab in chronic urticaria. A clinical case. J. Investig Allergol Clin Immunol 2010; 20(3): 252-254.
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