Grape seed extract (GSE) is a complex mixture of several compounds, mainly represented by polyphenols and phenolic acids . The aim of the present study was to determine the safe dose of GSE that can be used in the mouse model and to evaluate the potential toxicity that can be initiated by different concentrations of this substance. Forty-two mice were divided into 7 groups; groups 1 participated as controls, while animals in groups 2, 3, 4 were treated with 200, 400 and 800 mg/kg body weight of black grape seeds, respectively, and the remaining groups, including 5, 6 and 7 , were treated with 200, 400 and 800 mg/kg body weight of red grape seeds respectively. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay Animals were examined daily for any telltale signs of activity changes and toxicity along with body weight measurement throughout the experiment for 21 days. At the end of the study, the mice were euthanized, and the liver, kidneys, spleen, lungs, and brain were collected for histopathological examination. The results obtained from this study revealed that GSE doses of 200 and 400 mg/kg body weight were safe compared to the dose of 800 mg/kg body weight which resulted in reduced body weight gain and histopathological changes . No deaths occurred in all groups. Keywords: Albino mice, Black grape seeds, Red grape seeds; Sharbasher Village. Introduction: Plants are capable of producing a large number of chemical compounds with significant biological effects and have been used to produce a wide variety of medicines since the creation of man. Grapes, Vitis vinifera berries, are considered fruits of conventional value in the world. The primary composition of pomace is the skin, steam and seeds [4]. The chemical composition of fresh grapes is approximately 70-80% water and dissolved solids such as sugars, phenolic compounds, nitrogen compounds, organic acids, minerals, aromatic compounds, pectic substances. The unique combination of phytochemicals in grapes includes a variety of bioactive compounds such as simple phenols, flavonoids, anthocyanins, stilbenes, proanthocyanidins and vitamin E. Grape seeds are considered a waste product in industry and are made up of proteins, carbohydrates, lipids and 5-8% polyphenols depending on the type of grape. The composition of grape seeds differs significantly between different cultivars. Furthermore, multiple environmental and biological factors, such as light, drought, high salinity, cold, metal ions, pollutants, xenobiotics, toxins, experimental manipulations, pathogenic infections and plant aging can influence yields. and seed quality. A multitude of flavonoids are contained in GSE. The most abundant of these are proanthocyanidins, which are oligomers of flavan-3-ol monomeric units linked by carbon-carbon bonds. Please note: this is just a sample. Get a custom paper from our expert writers now. Get a custom essay Flavonoids are the most abundant biologically active phytonutrients among the polyphenols present in grapes, possessing cardioprotective, neuroprotective, antimicrobial, anti-aging, antioxidant, anti-inflammatory, anticancer and antimicrobial properties. Oral toxicity studies regarding the safety of grape seeds in experimental animals are lacking in the Kurdstan Region. Therefore, the main objective of this study was to determine the oral toxicity of a grape seed extract (black and red) extracted in water at different doses in mice, using.