1. Introduction1.1. Objective1.2. Theory and principles1.3. Application of the principles2. Experimental2.1. Table with the list of materials2.2. List of devices2.3. Procedure3. Data and calculations3.1. Recording of all relevant raw data3.2. Calculations with statistical manipulations4. Results and discussion4.1. Interpretation of data and comparison of results with known values4.2. Discussion on the meaning of the results4.3. Has the original objective been achieved?4.4. Conclusion5. References1. Introduction1.1. PurposeTo determine the kinetic parameters known as Vmax and Km of Alkaline Phosphatase. This will be done by determining and studying the optimal pH and temperature at which alkaline phosphatase functions.1.2. Theory and PrinciplesEnzymes are the most efficient catalysts known. (1) Enzymes are biomolecules that catalyze chemical reactions by decreasing the activation energy (the minimum energy required in a chemical system for a chemical reaction to occur), which in turn increases the rate of the chemical reaction. The catalyst itself does not change or wear out in the reactions it catalyzes. Enzymatic reactions consist of substrates and products. According to the Oxford Dictionary, a substrate is “the substance on which an enzyme acts” and a product is “a substance produced during a natural or chemical process”. (2) In general, all chemical reactions require enzymes to occur at a rate acceptable for life in biological systems. Enzymes are very substrate specific. This specificity of the enzyme molecule is due to the complementary shape of the active site of the protein and the substrate. An enzyme consists of two p...... in the center of the paper ......s compared to the pH on the x-axis, it was possible to determine the reaction rate at which the pH was at its maximum.4.2 . Discussion on the meaning of the results4.3. Has the original objective been achieved?4.4. Conclusion5. ReferencesWorks Cited1. Campbell MK, Farrell SO. Biochemistry. 7th edition: Brooks/Cole Cengage Learning; 2012.2. Oxford Dictionaries. Oxford Dictionaries. [Online].; 2014 [cited 19 April 2014. Available from: www.oxforddictionaries.com/definition/english/substrate.3. Mason KA, Losos JB, Singer SR. Biology. 10th ed. New York: McGraw Hill; 2014.4. AG Scientific. AG Scientific. [Online].; 2014 [cited April 19, 2014. Available from: www.agscientific.com/molecular-biology/molecular-biology.html.5. International Union of Biochemistry and Molecular Biology. [Online].; 2002 [cited April 21, 2014. Available from: onlinelibrary.wiley.com/store.